- Sheldon J. Plankton
- Dead Eye Plankton
- Mrs. Puff
- Incidental 155
- Incidental 107
- Incidental 105
- Incidental 46
- Justin Bottomfeeder (debut; mentioned)
In the beginning of the short, Plankton is introducing himself along with his evil genius diaries.
Plankton does not feel like himself and claims he thinks that the world knows him as an evil villain, and is more than just stealing the formula. He says that he has the voice of an angel, in which he then attempts to sing. He causes a window on a building to crack, as well as the windows of Mrs. Puff's Boating School to break and curious sunbathers to look up and wonder what the noises heard were.
Plankton finally appears and ends the short by saying that the world is not ready for a talent such as his, and starts to sing "The F.U.N. Song" poorly.
- Justin Bottomfeeder is a parody of Canadian pop singer Justin Bieber.