In "Fools in April," Mrs. Tentacles has a puffy white hair, a turquoise skin, and a long eyelashes, but no wrinkles. In "Krusty Towers," her first physical appearance, she has light purple hair, glasses, gold earrings, a pink gown and slippers, and looks older. This is also her current appearance.
Mrs. Tentacles seems to like baking cookies as seen in the episode "Krusty Towers." She also appears to be very friendly, although in "Fools in April" she was rude to Squidward in his hallucination. She is married to Jeff Tentacles, although it is presumed that the two are divorced as she appears to live alone and Jeff is shown to be alive in Hooray for Dads!
She makes her first live appearances in this episode because Squidward wants cookies like his mother used to make. When Mr. Krabs and SpongeBob arrive at her house to have her help them make the cookies, they appear as if they were about to rob or kidnap her, but she actually helps them pack up Mr. Krab's laundry. After Squidward gets his mother's cookies, Squidward replies "If only Mom was a better cook."
She appears in the bleachers during Squidward and Sandy's basketball game, where Sandy tells Squidward to take his complaining to his mother. He turns to his mother who tells him that she does not want to hear Squidward complaining about Sandy's basketball shot.