The cover features the five members of the Aquatic Adventurers, Mermaid Man, Barnacle Boy, Lighthouse Lass, the Green Harpoon, and the Flying Fish, sitting at a silver table. The title "The Aquatic Adventurers" is featured on the top, with the team's name in blue writing. The term "No. 1" appear in the top right corner of the cover, and the bottom of the cover has a sign saying that the comic stars the Aquatic Adventurers. According to the cover, the comic costed 10¢ when it was first published.
The story featured inside the comic is about the villain called the Invisible Isopod, and his plan to sneak into the Aquatic Adventurers' base, abduct them one by one, and make them his prisoners. However, the heroes break free of their binds and attack the Invisible Isopod, who is defeated shortly after.
Role in comic
When Mr. Krabs says that SpongeBob is not allowed to get autographs from the Aquatic Adventurers when they arrive at the Krusty Krab, SpongeBob pleads, saying that they have to sign his issue of Aquatic Adventurers No. 1, as no one has ever been able to get all five members to sign that issue. Mr. Krabs bans SpongeBob from getting autographs, but he himself plans to get the team to sign his own issue of the comic book, so he can sell it for a large profit.
When the Aquatic Adventurers arrive that night, Mr. Krabs drags the Flying Fish into his office to sign his copy of the comic book. Everyone else notice his disappearance, and SpongeBob compares it to the story in the comic book, where the Invisible Isopod abducts the heroes one by one. As Mr. Krabs gets Barnacle Boy, Lighthouse Lass, and the Green Harpoon to sign his copy, SpongeBob and the team fully believe that the Invisible Isopod is attacking them, so they hide in the bathroom.
As Mr. Krabs goes to get Mermaid Man's signature and finish the collection, he notices that everyone is gone. He hears something in the bathroom and opens the door, causing SpongeBob and the Aquatic Adventurers to attack him, thinking that he is the Invisible Isopod. After the attack, Barnacle Boy realizes that the Invisible Isopod was actually Mr. Krabs, who asked them to sign his copy. SpongeBob wonders why Mr. Krabs' would want the team to sign the comic book, before falsely thinking that Mr. Krabs was going to give the comic to him as a surprise. SpongeBob takes the comic and thanks Mr. Krabs as the latter groans in pain.